Comparison between Ocimum Sanctum Hepatoprotector Extract and Curcuma Xanthorrhiza on the Histological Structure of Aspartame-Induced Wistar Rats

Des Suryani, Humairah Medina Liza Lubis


The use of aspartame is still controversial, because there are studies stating that aspartame is safe to use, and there are studies suggesting aspartame has the potential to damage the liver, but aspartame has been approved by the FDA and BPOM in Indonesia with a daily dose of 50 mg / kg / day, the level of public knowledge we are still low to allow this dose to be overtaken, coupled with the presence of several food products that do not include the content of aspartame, basil leaves have been known to have hepatoprotector effect, but the dosage is still varied, and no researchers have compared curcuma Xanthoriza which is herbal medicine that has been quite well accepted in the community, Objective: to compare the hepatoprotector effect of basil leaf extract with xanthoriza curkuma, Method: Laboratory experimental study with posttest only with control group design. Wistar rats were divided into 5 groups and treated for 30 days. This study analyzed the histopathology of liver using paraffin histotechnics blocks, with HE staining and light microscopy. Analysis of degeneration degree using Kruskal-walis analysis post Hoc Mann-Whitney Results showed an increase in the degree of degeneration in the aspartame group at a dose of 100 mg / kgbb / day (p <0.05) compared to the normal group and treatment. Use of aspartame past the ADI dose damaged the liver, kurkuma and basil leaf extract at a dose of 300 mg / kg has the same protective effect on aspartame-induced rat liver, conclusions: aspartame is toxic for the liver, the use of basil leaf extract at a dose of 300 mg / kg / day or kurkuma xhantoriza extract can reduce the toxicity of aspartame.


xanthoriza; basil-curcuma; leaf; extracts

Full Text:



Ashok, I. et al. (2014) ‘Long-term effect of aspartame on the liver antioxidant status and histopathology in Wistar albino rats’, Biomedicine and Preventive Nutrition. Elsevier Masson SAS, 4(2), pp. 299–305. doi: 10.1016/j.bionut.2013.10.002.

Asif, M. (2013) ‘Low Caloric Sweeteners for Diabetes and Obesity Care and Their Clinical Inferences for Tackle the Prevalence’, (5).

Azad, M. B. et al. (2017) ‘Nonnutritive sweeteners and cardiometabolic health: A systematic review and meta-analysis of randomized controlled trials and prospective cohort studies’, Cmaj, 189(28), pp. E929–E939. doi: 10.1503/cmaj.161390.

Haliem, N. G. A. El and Mohamed, D. S. (2011) ‘The effect of aspartame on the histological structure of the liver and renal cortex of adult male albino rat and the possible protective effect of Pimpinella anisum oil’, pp. 10–20.

doi: 10.1097/01.EHX.0000406589.05585.8d.

Iyaswamy, A. et al. (2017) ‘Disruption of redox homeostasis in liver function and activation of apoptosis on consumption of aspartame in folate deficient rat model’, Journal of Nutrition & Intermediary Metabolism. Elsevier Inc, 8, pp. 41–50.

doi: 10.1016/j.jnim.2017.06.002.

Jain, A. (2015) ‘To Evaluate Hepatoprotective Activity of Leaves of Ocimum Sanctum Using Animal Model’, 8(4), pp. 4–7.

Kumar Choudhary, A., Selvaraj, S. and Sheela Devi, R. (2015) ‘Aspartame Induce Modification in Membrane Bound and Antioxidant Enzymes in Liver and Kidney of Wistar Albino Rats’, Current Nutrition & Food Science, 10(4), pp. 275–287.

doi: 10.2174/1573401310666141024215431.

Leon, A. S. et al. (1989) ‘Safety of Long-term Large Doses of Aspartame’, Archives of Internal Medicine, 149(10), pp. 2318–2324. doi: 10.1001/archinte.1989.00390100120026.

Masoud, R. E. (2017) ‘Hepatoprotective Effect of Curcumin Versus Silymarin on’, 8(2), pp. 134–141.

Menteri, P. and Republik, K. (2009) ‘Bahaya bahan tambahan makanan bagi kesehatan’, 5(1), pp. 16–22.

Mourad, M. (2011) ‘Effect of aspartame on some oxidative stress parameters in liver and kidney of rats’, 5(June), pp. 678–682.

Palipoch, S. et al. (2014) ‘Hepatoprotective effect of curcumin and alpha-tocopherol against cisplatin-induced oxidative stress’, BMC Complementary and Alternative Medicine, 14, pp. 1–8. doi: 10.1186/1472-6882-14-111.

Prokić, Marko D. et al. (2015) ‘Effect of aspartame on biochemical and oxidative stress parameters in rat blood’, Archives of Biological Sciences, 67(2), pp. 535–545.

doi: 10.2298/ABS141009016P.


doi: 10.2298/ABS141009016P.

Rahman, shahedur (2011) ‘Ocimum sanctum L Phytochemical and pharmacological Profile’.

Shah, K. and Verma, R. J. (2012) ‘Protection against butyl p-hydroxybenzoic acid induced oxidative stress by ocimum sanctum extract in mice liver’, Acta Poloniae Pharmaceutica - Drug Research, 69(5), pp. 865–870.



  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.