Test the Cardioprotective Effectiveness of Turmeric Ethanol Extract (Curcuma Longa) in Doxorubicin-In-Rat Wistar (Rattus Norvegicus) Doxorubicin-Induced Males

Wang Xiqin, Florenly Florenly, Liena Liena, Johannes Bastira

Abstract


Cardiotoxicity is mostly due to the use of chemotherapy drugs, with the class of anthracyclines drugs being 86.8. One of the most commonly used anthracyclines is doxorubicin. Turmeric with the scientific name Curcuma longa linn is one of the medicinal plants, the main compound is curcumin is considered able to reduce the risk of heart attack. The goal of this study tested the cardioprotective effectiveness of turmeric ethanol extract (curcuma longa linn.) in doxorubicin-induced male wistar (rattus norvegicus) mice. This type of research is experimental, in the Pharmacology Laboratory of the Faculty of Pharmacy, University of North Sumatra, and November 2020. Animals experimented on male wistar rats (Rattus norvegicus) weighing 150 - 200 grams. Data analysis, if (P > 0.05), continued with One Way ANOVA, but if (P <.05) followed by the Tukey HSD Post Hoc test to see real differences between treatments. If the distributed data is abnormal then the Kruskal-Wallis test results are used. Statistical test results, serum levels of CK–MB in the normal group have significant differences (p<0.05) with the negative control treatment group and the treatment group I, but do not have a significant difference (p>0.05) with the positive control group, treatment group II and the treatment group III. Concluding that turmeric ethanol extract has the ability to lower levels of biomarkers of heart damage namely CK-MB and LDH induced with doxorubicin, gradually increasing doses of turmeric ethanol extract can reduce the incidence of heart cell damage, which is most effective at doses of EEK 500 mg / kg BB. Turmeric ethanol extract has cardioprotective effects induced with doxorubicin. Administration of turmeric ethanol extract can repair heart organ tissue induced with doxorubicin based on histological picture of the heart. It is hoped that researchers will be able to conduct research on the toxic effects of giving turmeric ethanol extract to the hearts of mice conducted subchronically.


Keywords


turmeric ethanol extract; cardioprotective; doxorubicin

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References


Ariani, S. 2017. Stop Kanker, Cara Praktis Olah Tanaman Obat Kanker. Yogyakarta: Istana Media.

Barui, Sugata, Soumen Saha, Goutam Mondal, Shaik Haseena, and Arabinda Chaudhuri. 2014. “Simultaneous Delivery of Doxorubicin and Curcumin Encapsulated in Liposomes of Pegylated RGDK-Lipopeptide to Tumor Vasculature.” Biomaterials 35(5):1643–56.

Biogen. 2020. “Aducanumab for the Treatment of Alzheimer’s Disease.” U.S. Food & Drug Administration Peripheral and Central Nervous System Drugs Advisory Committee (October).

Chakraborty, Manodeep, Ananya Bhattacharjee, and Jagadish Vasudev Kamath. 2017. “Cardioprotective Effect of Curcumin and Piperine Combination against Cyclophosphamide-Induced Cardiotoxicity.” Indian Journal of Pharmacology 49(1):65–70.

Chattopadhyay, Ishita, Kaushik Biswas, Uday Bandyopadhyay, and Ranajit K. Banerjee. 2004. “Turmeric and Curcumin: Biological Actions and Medicinal Applications.” Current Science 87(1):44–53.

Childs, April C., Sharon L. Phaneuf, Amie J. Dirks, Tracey Phillips, and Christiaan Leeuwenburgh. 2002. “Doxorubicin Treatment in Vivo Causes Cytochrome c Release and Cardiomyocyte Apoptosis, as Well as Increased Mitochondrial Efficiency, Superoxide Dismutase Activity, and Bcl-2:Bax Ratio.” Cancer Research 62(16):4592–98.

Duan, Jinghua, Heidi M. Mansour, Yangde Zhang, Xingming Deng, Yuxiang Chen, Jiwei Wang, Yifeng Pan, and Jinfeng Zhao. 2012. “Reversion of Multidrug Resistance by Co- Encapsulation of Doxorubicin and Curcumin in Chitosan/Poly(Butyl Cyanoacrylate) Nanoparticles.” International Journal of Pharmaceutics 426(1–2):193–201.

El-Sayed, Eman M., Amal S. Abd El-azeem, Abeer A. Afify, Manal H. Shabana, and Hanaa H. Ahmed. 2011. “Cardioprotective Effects of Curcuma Longa L. Extracts against Doxorubicin-Induced Cardiotoxicity in Rats.” Journal of Medicinal Plants Research 5(17):4049–58.

Han, Xiuzhen, Jihong Pan, Dongmei Ren, Yanna Cheng, Peihong Fan, and Hongxiang Lou.

“Naringenin-7-O-Glucoside Protects against Doxorubicin-Induced Toxicity in H9c2 Cardiomyocytes by Induction of Endogenous Antioxidant Enzymes.” Food and Chemical Toxicology 46(9):3140–46.

Hartati, Sri Yuni. 2013. “Perkebunan_KhasiatKunyit.Pdf.” Warta Penelitian Dan Pengembangan Tanaman Industri 19(2):5.

Huang, Chengqun, Xiaoxue Zhang, Jennifer M. Ramil, Shivaji Rikka, Lucy Kim, Youngil Lee, Natalie A. Gude, Patricia A. Thistlethwaite, Mark A. Sussman, Roberta A. Gottlieb, and Åsa

B. Gustafsson. 2011. “NIH Public Access.” 121(5):675–83.

Imbaby, S., M. Ewais, S. Essawy, and N. Farag. 2014. “Cardioprotective Effects of Curcumin and Nebivolol against Doxorubicin-Induced Cardiac Toxicity in Rats.” Human and Experimental Toxicology 33(8):800–813.

Justyna, Widomska. 2017. “乳鼠心肌提取 {HHS} {Public} {Access}.” Physiology & Behavior 176(5):139–48.

Martha, Januar Wibawa, Sunarya Surianata, and Amiliana Santoso. 2007. “Left Ventricle Diastolic Function in Patients Underwent Chemotherapy with Doxorubicin Gambaran Fungsi Diastolik Ventrikel Kiri Pada Penderita Keganasan Yang Mendapat Kemoterapi Doxorubicin.” 28(5):320–26.

Minotti, Giorgio, Stefania Recalcati, Alvaro Mordente, Giovanni Liberi, Antonio Maria Calafiore, Cesare Mancuso, Paolo Preziosi, and Gaetano Cairo. 1998. “The Secondary Alcohol Metabolite of Doxorubicin Irreversibly Inactivates Aconitase/Iron Regulatory Protein‐1 in Cytosolic Fractions from Human Myocardium.” The FASEB Journal 12(7):541–52.

Mohamad, Ragaa Hosny, Amal Mohamad El-Bastawesy, Zekry Khalid Zekry, Hussain A. Al- Mehdar, Mohamad Gamil Abdel Monaam Al-Said, Soaad Shaker Aly, Sabry Mohamed Sharawy, and Mahmuod M. El-Merzabani. 2009. “The Role of Curcuma Longa against Doxorubicin (Adriamycin)-Induced Toxicity in Rats.” Journal of Medicinal Food 12(2):394–402.

Nabofa, Williams E. E., Oluwadamilola O. Alashe, Oyetunde T. Oyeyemi, Alfred F. Attah, Ademola A. Oyagbemi, Temidayo O. Omobowale, Adeolu A. Adedapo, and Akinola R. A. Alada. 2018. “Cardioprotective Effects of Curcumin-Nisin Based Poly Lactic Acid Nanoparticle on Myocardial Infarction in Guinea Pigs.” Scientific Reports 8(1):1–11.

Octavia, Yanti, Carlo G. Tocchetti, Kathleen L. Gabrielson, Stefan Janssens, Harry J. Crijns, and An L. Moens. 2012. “Doxorubicin-Induced Cardiomyopathy: From Molecular Mechanisms to Therapeutic Strategies.” Journal of Molecular and Cellular Cardiology 52(6):1213–25.

Olson, R. D., P. S. Mushlin, D. E. Brenner, S. Fleischer, B. J. Cusack, B. K. Chang, and R. J. Boucek. 1988. “Doxorubicin Cardiotoxicity May Be Caused by Its Metabolite, Doxorubicinol.” Proceedings of the National Academy of Sciences of the United States of America 85(10):3585–89.

Sabale, Prafulla, Arjun Modi, and Vidya Sabale. 2013. “Curcuma Longa Linn. A Phytochemical and Phytopharmacological Review.” Research Journal of Pharmacognosy and Phytochemistry 5(2):59–68.

Schunke, Kathryn J., Luke Coyle, Gary F. Merrill, and David T. Denhardt. 2013. “Acetaminophen Attenuates Doxorubicin-Induced Cardiac Fibrosis via Osteopontin and GATA4 Regulation: Reduction of Oxidant Levels.” Journal of Cellular Physiology 228(10):2006–14.

Siahaan, Irwan Harpen, Tina Christina Tobing, Nelly Rosdiana, and Bidasari Lubis. 2016. “Dampak Kardiotoksik Obat Kemoterapi Golongan Antrasiklin.” Sari Pediatri 9(2):151–56.

Tacar, Oktay, Pornsak Sriamornsak, and Crispin R. Dass. 2013. “Doxorubicin: An Update on Anticancer Molecular Action, Toxicity and Novel Drug Delivery Systems.” Journal of Pharmacy and Pharmacology 65(2):157–70.

Thomas, S. A. A. 2017. “Chemotherapy Agents That Cause Cardiotoxicity.” US Pharm 42(9):24–33.

Wanninger, Simon, Volker Lorenz, Abdus Subhan, and Frank T. Edelmann. 2015a. “Metal Complexes of Curcumin - Synthetic Strategies, Structures and Medicinal Applications.” Chemical Society Reviews 44(15):4986–5002.

Wanninger, Simon, Volker Lorenz, Abdus Subhan, and Frank T. Edelmann. 2015b. “Metal Complexes of Curcumin - Synthetic Strategies, Structures and Medicinal Applications.” Chemical Society Reviews 44(15):4986–5002.

Wattanapitayakul, Suvara K., Linda Chularojmontri, Angkana Herunsalee, Suphan Charuchongkolwongse, Somchit Niumsakul, and John A. Bauer. 2005. “Screening of Antioxidants from Medicinal Plants for Cardioprotective Effect against Doxorubicin Toxicity.” Basic and Clinical Pharmacology and Toxicology 96(1):80–87.

Wisman, Beta Agustia, Sally Aman Nasution, and Marulam M. Panggabean. 2017. “Kardiotoksisitas Akibat Terapi Radiasi (Cardiotoxicity Complication of Radiotherapy).” Jurnal Penyakit Dalam Indonesia 4(4):214–23.

Zhou, Shaoyu, Anatoli Starkov, M. Kent Froberg, Richard L. Leino, and Kendall B. Wallace. 2001. “Cumulative and Irreversible Cardiac Mitochondrial Dysfunction Induced by Doxorubicin.” Cancer Research 61(2):771–77.




DOI: https://doi.org/10.33258/birex.v3i4.2661

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