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Detection of Enterobacteriaceae producing Extended Spectrum -Lactamases (ESBL) in Beef Cattle Sold at the Animal Market in Central Lombok
Abstract
The Province of West Nusa Tenggara Province (NTB) is known as a source of beef cattle in eastern Indonesia. The use of antibiotics in animal foods can threaten health and cause antibiotic resistance to the normal flora of the animal and humans. One of the causes of antibiotic resistance is extended spectrum β-lactamase (ESBL). The purpose of the study was to detect the prevalence of ESBL-producing Enterobacteriaceae in beef cattle sold in the central Lombok animal market. The faecal samples were collected from beef cattle to 65. Cultured on MacConkey agar supplemented with cefotaxim 1 mg/L, incubated at 37oC for 24 hours. Then the growing colony were tested for ESBL producer by Double Disk Synergy Test (DDST). Identify the type of bacteria by biochemical testing. Identify the type of bacteria by biochemical testing and gram staining. Results: identified showed 1 (1.5%) sample of ESBL positive beef cattle the faecal samples with Escherichia coli bacteria (100%). Through feces, resistant bacteria contained in animal faecal can migrate to the environment and cross-transmission of genetic material between bacteria through water, soil, air, plants, food, humans and food-producing animals.
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Aniza, S, N, Ary Andini, Indah Lestari. 2019. Analisis residu antibiotik tetrasiklin pada Daging ayam broiler dan daging sapi. Jurnal Sain Health. 3(2).
Bahri S, Masbulan E, Kusumaningsih A. 2005. Proses Praproduksi sebagai Faktor Penting dalam Menghasilkan Produk Ternak yang Aman untuk Manusia. Jurnal Litbang Pertanian 24 (1)
Departemen Pertanian Badan Penelitian dan Pengembangan Pertanian Balai Pengkajian Teknologi Pertanian. 2003. Petunjuk Teknis Manajemen Terpadu Pemeliharaan Sapi Bali. 36-38.
Dewi, A.A.S., Widdhiasmoro, N.P., Nurlatifah, I., Riti, N., Purnawati, D. 2014. Residu Antibiotika pada Pangan Asal Hewan, Dampak dan Upaya Penanggulangannya. Buletin Veteriner, BBVet Denpasar. Vol. XXVI (85)
Effendi, M H, Risi C, Jola Rahmahani, Wiwiek Tyasningsih.2020. Public Awareness for Antimicrobial Resistance from Escherichia coli Isolated from Beef Sold on Several Wet Market in Surabaya, Indonesia. Indian Journal of Public Health Research & Development. Vol. 11, No. 9. 295-300
Hiroi, Midori. Fumie Yamazaki, Tetsuya Harada, Naomi Takahashi, Natsuko Iida, Yoshihiro Noda, Miya Yagi, Tomohiro Nishio, Takashi Kanda, Fumihiko Kawamori, Kanji Sugiyama, Takashi Masuda, Yukiko Hara-kudo and Norio Ohashi. 2011. Prevalence of Extended-Spectrum -Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Food-Producing Animals. J.Vet. Med. Sci. 74(2). 189–195.
Ilham, N. 2001. Prospek Pasar dan Sistem Tataniaga Ternak dan Daging Sapi di Nusa Tenggara Barat. Wartazoa .Buletin Ilmu Peternakan Indonesia. 11(2):32-43
Imasari T, Wiwiek T, Eddy B W, Kuntaman K. 2018. Prevalensi dan Pola Gen Extended Spectrum -lactamaseBakteri Usus Sapi Perah dan PendudukSekitar Peternakan di Surabaya. Jurnal Veteriner. 19(3). 313-320.
Kuntaman.2018. The Prevalence Of Extended Spectrum -Lactamase (ESBL) Producing Gut Bacterial Flora among Patients in Dr. Soetomo Hospital and Primary Health Center in Surabaya. Jurnal Folia Medica Indonesia, 54(4). 256-262
Lindawati A, Priyo B, et al. 2015. Buku Ajar Pemeriksaan Mikrobiologi Pada Penyakit Infeksi. Sagung Seto.
Naelasari, DN, Eko B K, Linda Dewanti, Sulistiawati, Rosantia Sarassari, K.
Normaliska, Ratna. Mirnawati Bachrum Sudarwanto, Hadri Latif. 2019. Pola Resistensi Antibiotik pada Escherichia coli Penghasil ESBL dari Sampel Lingkungan di RPH-R Kota Bogor. Jurnal Acta Veterinaria Indonesiana. Vol. 7(2). 42-48.
Odenthal, S., Akineden O., Usleber. 2016. Extended-Spectrum -Lactamases Producing Enterobacteriaceae in Bulk Tank Milk from German Dairy Farms. International Journal of Food Microbiology. Vol.238. 72-78.
Putri, Indriyanti Marselina. 2021. Kinerja Ekonomi Usaha Ternak Sapi Potong Di Wilayah Penerima Program 1000 Desa Sapi Di Kecamatan Pujut Kabupaten Lombok Tengah. Skripsi.
Sarassari Rosantia. 2017. Perbandingan pola fenotipik dan genotipik bakteri penghasil ESBL dari flora usus pasien di ICU, Rawat Inap Tropik Infeksi, Dan Populasi Di Komunitas. An unpublished thesis. Surabaya, Faculty of Medicine, Universitas Airlangga.
Soedarto. 2016. Hospital Nosocomial Infections. Infeksi Nosokomial Di Rumah Sakit.Edisi Pertama, Cetakan Pertama. Surabaya: Penerbit Sagung Seto
Sukmawinata E. 2015. Tingkat kejadian Escherichia coli penghasil Extended Spectrum β-Lactamase di feses sapi di rumah potong hewan ruminansia Kota Bogor. Tesis S2. Sekolah Pascasarjana, Institut Pertanian Bogor. Bogor.
Suryana, ES, Martiano D, Baliwati, YF. 2019. Pola Konsumsi dan Permintaan Pangan Sumber Protein Hewani di Provinsi Nusa Tenggara Barat. Jurnal Analisis Kebijakan Pertanian.17 (1); 1-12
Syahputra A R, Effendi M H, Setiawan K, Suwarno , Wiwik T, Agnes T S E. 2019. Identifikasi Bakteri Escherichia coli Penghasil Extended Spectrum β-Lactamase Dari Swab Rectal Sapi Perah Menggunakan Metode Vitek-2 Di Kud Tani Wilis Sendang Kabupaten Tulungagung. Journal of Basic Medicine Veterinary. Vol.8 (2). 108-114.
Umadevi S, Kandhakumari G, Joseph N M, Kumar S, Easow J M, Stephen S, Singh U K. 2011. Prevalence and antimicrobial susceptibility pattern of ESBL producing Gram NegativeBacilli. Journal of Clinical and Diagnostic Research. 5(2). 236-239.
WittumTE, Mollenkopf DF, Daniels JB, Parkinson AE Mathews JL, Fry PR, AbleyMJ, Gebreyes WA. 2010. CTXM-typeexpended-spectrum b-lactamases present inEscherichia coli from the feces of cattle inOhio, United States. Journal Foodborne Pathog Dis. vol 7. 1575-1579
DOI: https://doi.org/10.33258/birex.v4i2.4595
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